I use what I have simply called an adjustable knot, although, I have been informed that it is actually a tautline hitch... either way, it works great IMO. If the cord is a bit slippery, or the wind is really acting up, just throw an extra wrap in the original knot, or throw a half hitch behind it... Hasn't let me down yet...
I made a quick little video showing the knot here: Tying an Adjustable Knot
As for stakes, I love my shepherd hooks, and think that the ones that Lawson Kline sells are some really good ones...
If you want your line to glow, check out Lawson's GloWire. It comes in a few different colors, and is not super small so it is easy to work with.
Check out the stakes and the GloWire here: Lawson Outdoor Equipment
If you like your line a little thinner/lighter, the GLine that LiteTrail has is really nice stuff. It doesn't glow, but it is nice and strong, and even a little stiff, which makes working with it a little easier... Check it out here: LiteTrail He also makes the same stuff in a slightly larger diameter too... it's on the site.
If you like the thin stuff, but also want some that glows, my favorite is the "Dyneema rope with reflective tracer" from Picharpak Workshop. Check it out here: Picharpak Workshop
Hope this helps some!